AKT1 Knockout Cell Line - CD BioSciences

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AKT1 Knockout Cell Line

AKT1 Knockout Cell Line

SPL-00183

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Description
5bp deletion
Target Information
Target Name Akt
Gene Abbr. AKT1
Gene ID 207
Full Name AKT serine/threonine kinase 1
Alias AKT, PKB, PKB-ALPHA, PRKBA, RAC
Species Human
Genomic Locus chr14:104775722
Transcript NM_001014431
WT Expression Level 174.79 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 5bp deletion in a coding exon of AKT1.
Description 5bp deletion
Parental Cell Line C631
Guide RNA Sequence GAGGAGATGGACTTCCGGTC
PCR Primer Forward: CCTACATGGAAAACCGGCCTA
Reverse: GACAACCGCCATCCAGACT
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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