AKT1 (-/-); AKT2 (-/-) Cell Line - CD BioSciences

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AKT1 (-/-); AKT2 (-/-) Cell Line

AKT1 (-/-); AKT2 (-/-) Cell Line

SPL-00196

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Description
AKT1 (-/-); AKT2 (-/-)
Target Information
Target Name Akt
Gene Abbr. AKT2
Gene ID 208
Full Name AKT serine/threonine kinase 2
Alias HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
Species Human
Genomic Locus 14q32.32;19q13.1-q13.2
Introduction This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HCT116
Genotype AKT1 (-/-); AKT2 (-/-)
Description AKT1 (-/-); AKT2 (-/-)
Parental ATCC ID CCL-247
STR Profile Amelogenin: X,Y; CSF1PO: 7,10; D13S317: 10,12; D16S539: 11,13; D5S818: 10,11; D7S820: 11,12; THO1: 8,9; TPOX: 8,9; vWA: 17,22; ATCC
Disease Colorectal carcinoma
Tissue Colon
Gender Male
Key Endogenous Mutations CDKN2A, CTNNB1, KRAS, PIK3CA, MLH1
CVT Selection Condition G418 resistant - 0.3 mg/mL
Cell Number 1x10^6
Handling Specifications
Revival Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20.
Culture Medium RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20.
Freeze Medium Complete growth media 45% + USDA approved FBS 50% + DMSO 5%
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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