AKT1 (-/-); AKT2 (-/-) Cell Line - CD BioSciences

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AKT1 (-/-); AKT2 (-/-) Cell Line

AKT1 (-/-); AKT2 (-/-) Cell Line

SPL-00185

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Description
AKT1 (-/-); AKT2 (-/-)
Target Information
Target Name Akt
Gene Abbr. AKT1
Gene ID 207
Full Name AKT serine/threonine kinase 1
Alias AKT, PKB, PKB-ALPHA, PRKBA, RAC
Species Human
Genomic Locus 14q32.32;19q13.1-q13.2
Introduction The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011].
Product Details
Cell Line Model DLD-1
Genotype AKT1 (-/-); AKT2 (-/-)
Description AKT1 (-/-); AKT2 (-/-)
Parental ATCC ID CCL-221
STR Profile Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 8,11; D16S539: 12,13; D5S818: 13; D7S820: 10,12; THO1: 7,9.3; TPOX: 8,11; vWA: 18,19; ATCC
Disease Colorectal adenocarcinoma, Dukes Type C
Tissue Colon
Gender Male
Key Endogenous Mutations APC, BRCA2, FAM123B, KRAS, MSH6, PI3KA, TP53,
CVT Selection Condition G418 resistant - 1 mg/mL
Cell Number 1x10^6
Handling Specifications
Revival Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:6-1:10.
Culture Medium RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:6-1:10.
Freeze Medium Complete growth media 45% + USDA approved FBS 50% + DMSO 5%
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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