AKAP9 Knockout Cell Line - CD BioSciences

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AKAP9 Knockout Cell Line

AKAP9 Knockout Cell Line

SPL-00176

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Description
10bp deletion
Target Information
Target Name AKAP9
Gene Abbr. AKAP9
Gene ID 10142
Full Name A-kinase anchoring protein 9
Alias AKAP-9, AKAP350, AKAP450, CG-NAP, HYPERION
Species Human
Genomic Locus chr7:92001156
Transcript NM_005751
WT Expression Level 9.27 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq, Aug 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 10bp deletion in a coding exon of AKAP9.
Description 10bp deletion
Parental Cell Line C631
Guide RNA Sequence GTACTATATCAGTTTCGAAC
PCR Primer Forward: TGAAGCAGTATAATTTGCCAGAAGC
Reverse: CCTTAAAGCATTCTCCATTTCTCCC
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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