AKAP11 Knockout Cell Line - CD BioSciences

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AKAP11 Knockout Cell Line

AKAP11 Knockout Cell Line

SPL-00166

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Description
2bp insertion
Target Information
Target Name AKAP11
Gene Abbr. AKAP11
Gene ID 11215
Full Name A-kinase anchoring protein 11
Alias AKAP-11, AKAP220, PPP1R44, PRKA11
Species Human
Genomic Locus chr13:42298628
Transcript NM_016248
WT Expression Level 7.75 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed at high levels throughout spermatogenesis and in mature sperm. It binds the RI and RII subunits of PKA in testis. It may serve a function in cell cycle control of both somatic cells and germ cells in addition to its putative role in spermatogenesis and sperm function. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 2bp insertion in a coding exon of AKAP11.
Description 2bp insertion
Parental Cell Line C631
Guide RNA Sequence TACTGGTATAAGGTACACCT
PCR Primer Forward: TTACAAAACCAAGGAGCTTTTCAGG
Reverse: GGCTTTGAAGTCTCTTCCTCTTCTA
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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