AKAP1 Knockout Cell Line - CD BioSciences

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AKAP1 Knockout Cell Line

AKAP1 Knockout Cell Line

SPL-00163

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Description
2bp deletion
Target Information
Target Name AKAP1
Gene Abbr. AKAP1
Gene ID 8165
Full Name A-kinase anchoring protein 1
Alias AKAP, AKAP121, AKAP149, AKAP84, D-AKAP1
Species Human
Genomic Locus chr17:57105807
Transcript NM_003488
WT Expression Level 109.07 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 2bp deletion in a coding exon of AKAP1.
Description 2bp deletion
Parental Cell Line C631
Guide RNA Sequence ACAGACATGAGATTGCGACC
PCR Primer Forward: GAAGACGTCTGTCCCAAAGTAGT
Reverse: GCTGATTTGCTGGAGAATAGTACAC
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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