AIF Antibody - CD BioSciences

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AIF Antibody

AIF Antibody

SPA-00279

Size Price
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100 µg Online Inquiry
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Target Information
Target Name AIF
Gene Abbr. AIFM1
Gene ID 9131
Full Name apoptosis inducing factor mitochondria associated 1
Alias AIF, AUNX1, CMT2D, CMTX4, COWCK
Introduction Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli. Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis. Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain. While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis. Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity.
Product Details
Host Goat
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Peptide with sequence C-DDPNVTKTLRFKQ corresponding to internal region according to NP_004199.1, NP_665811.1, NP_001124319.1.
Usage
Application WB, IF, ELISA
Dilutions Western Blot (0.1-0.3 µg/mL); Immunofluorescence (10 µg/mL); ELISA (1:128000)
MW(KDa) 57, 67
Reactivity Human, Mouse, Bovine
Specificity This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).
Storage & Handling
Storage Buffer Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA.
Preservative 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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