AGO1 Knockout Cell Line - CD BioSciences

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AGO1 Knockout Cell Line

AGO1 Knockout Cell Line

SPL-00140

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Description
11bp deletion
Target Information
Target Name AGO1
Gene Abbr. AGO1
Gene ID 26523
Full Name argonaute RISC component 1
Alias EIF2C, EIF2C1, GERP95, Q99, hAgo1
Species Human
Genomic Locus chr1:35888462
Transcript NM_012199
WT Expression Level 10.40 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 11bp deletion in a coding exon of AGO1.
Description 11bp deletion
Parental Cell Line C631
Guide RNA Sequence TTCCAGGCACCTCGCCGGCC
PCR Primer Forward: CTCTTTCAAGGTTGGTGTCTTTCAG
Reverse: GCATTCTCTATACTCTCGTGTTCCT
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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