ADRA1A Knockout Cell Line - CD BioSciences

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ADRA1A Knockout Cell Line

ADRA1A Knockout Cell Line

SPL-00119

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Description
5bp deletion
Target Information
Target Name Adrenergic Receptor
Gene Abbr. ADRA1A
Gene ID 148
Full Name adrenoceptor alpha 1A
Alias ADRA1C, ADRA1L1, ALPHA1AAR
Species Human
Genomic Locus chr8:26864684
Transcript NM_000680
WT Expression Level 0.08 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 5bp deletion in a coding exon of ADRA1A.
Description 5bp deletion
Parental Cell Line C631
Guide RNA Sequence TGCCGAAGGCCCAGTAGCCT
PCR Primer Forward: GAACAGGGGTCCAATGGATATGA
Reverse: TAACATCCTAGTGATCCTCTCCGTA
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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