ADIPOR1 Knockout Cell Line - CD BioSciences

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ADIPOR1 Knockout Cell Line

ADIPOR1 Knockout Cell Line

SPL-00108

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Description
5bp deletion
Target Information
Target Name ADIPOR
Gene Abbr. ADIPOR1
Gene ID 51094
Full Name adiponectin receptor 1
Alias ACDCR1, CGI-45, CGI45, PAQR1, TESBP1A
Species Human
Genomic Locus chr1:202946534
Transcript NM_015999
WT Expression Level 176.84 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene encodes a protein which acts as a receptor for adiponectin, a hormone secreted by adipocytes which regulates fatty acid catabolism and glucose levels. Binding of adiponectin to the encoded protein results in activation of an AMP-activated kinase signaling pathway which affects levels of fatty acid oxidation and insulin sensitivity. A pseudogene of this gene is located on chromosome 14. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2014].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 5bp deletion in a coding exon of ADIPOR1.
Description 5bp deletion
Parental Cell Line C631
Guide RNA Sequence GACAACGACTATCTGCTACA
PCR Primer Forward: ACTTTGTTGGGCTCTATAATCCCTT
Reverse: CTATTACCTAGGACCATTCAGAGCC
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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