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ADAMTS8 Antibody
SPA-00233
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ADAMTS8 |
| Gene Abbr. | ADAMTS8 |
| Gene ID | 11095 |
| Full Name | ADAM metallopeptidase with thrombospondin type 1 motif 8 |
| Alias | ADAM-TS8, METH2 |
| Introduction | ADAMTS8 (A disintegrin and metalloprotease with thrombospondin motifs 8; also METH-2) is a 95 kDa member of an ADAMTS subfamily of Zn metalloproteases that includes ADAMST-1, -4, -5 and -15. It is expressed by chrondrocytes, neurons, astrocytes and macrophages, and likely participates in proteoglycan (aggrecan) proteolysis. Human proADAMTS8 is a secreted, 863 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 27-213), a peptidase M12B domain (aa 219-429), a disintegrin region (aa 438-525), and two TSP type I sequences (aa 526-888) that are separated by an intervening spacer domain (aa 690-831). Cleavage of the proregion generates a mature 80 kDa molecule that may undergo additional processing to create a 65-67 kDa truncated form. There are two potential splice variants. One shows a 31 aa substitution for aa 411-889, while another shows an alternative start site at Met231. Over aa 29-691, human ADAMTS8 shares 79% aa identity with mouse ADAMTS8. |
| Product Details | |
|---|---|
| Host | Sheep |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS8. Pro29-Arg691 (Gly35Arg, Gly431Ala, Val526Ala). Accession # AAD48081. |
| Usage | |
|---|---|
| Application | WB, IHC, IP |
| Dilutions | Western Blot (1 µg/mL); Immunohistochemistry (5-15 µg/mL); Immunoprecipitation (25 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human ADAMTS8 in direct ELISAs and Western Blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ADAMTS1 is observed and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS3, rhADAMTS4, rhADAMTS5, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.