ACTN4 Knockout Cell Line - CD BioSciences

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ACTN4 Knockout Cell Line

ACTN4 Knockout Cell Line

SPL-00076

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Description
7bp deletion
Target Information
Target Name ACTN4
Gene Abbr. ACTN4
Gene ID 81
Full Name actinin alpha 4
Alias ACTININ-4, FSGS, FSGS1
Species Human
Genomic Locus chr19:38647847
Transcript NM_004924
WT Expression Level 149.18 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 7bp deletion in a coding exon of ACTN4.
Description 7bp deletion
Parental Cell Line C631
Guide RNA Sequence CCCGGTCCCAGTCGTCCTCC
PCR Primer Forward: CTGGTGGGCGAGCGAGAG
Reverse: GCCAATTCTCTTTTCAGATTCCCAA
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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