Acetyl-Histone H3 (Lys14/Lys9) Antibody - CD BioSciences

service-banner

Acetyl-Histone H3 (Lys14/Lys9) Antibody

Acetyl-Histone H3 (Lys14/Lys9) Antibody

SPA-05149

Size Price
50 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name Histone H3
Gene Abbr. H3C14
Gene ID 126961
Full Name H3 clustered histone 14
Alias H3, H3.2, H3/M, H3C13, H3C15
Introduction Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen The exact sequence of the immunogen to this Histone H3 [ac Lys14, ac Lys9] antibody is proprietary.
Usage
Application WB, ChIP, ELISA, IF
Dilutions Western Blot (1:1000); Chromatin Immunoprecipitation (1-2 μg/IP); Dot Blot (1:20000); ELISA (1:100); Immunofluorescence (1:500); Immunofluorescence (1:500)
MW(KDa) 17
Reactivity Human, Mouse, A. thaliana, Fungi, Zebrafish
Storage & Handling
Storage Buffer PBS.
Preservative 0.05% Sodium Azide and 0.05% ProClin 300
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.