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Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody
SPA-01484
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CBP/p300 |
| Gene Abbr. | CREBBP |
| Gene ID | 1387 |
| Full Name | CREB binding protein |
| Alias | CBP, KAT3A, MKHK1, RSTS, RSTS1 |
| Introduction | CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery. CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins. Phosphorylation of p300 at Ser89 by PKC represses its transciptional acitivity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors. Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ. Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex. CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS. The role of acetylation of CBP/p300 is of particular interest. Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IP, ChIP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 300 |
| Reactivity | Human, Mouse, Monkey |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of CBP or p300 only when acetylated at lysine 1535 or lysine 1499, respectively. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.