ABL2 Knockout Cell Line - CD BioSciences

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ABL2 Knockout Cell Line

ABL2 Knockout Cell Line

SPL-00041

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Description
10bp deletion
Target Information
Target Name c-Abl
Gene Abbr. ABL2
Gene ID 27
Full Name ABL proto-oncogene 2, non-receptor tyrosine kinase
Alias ABLL, ARG
Species Human
Genomic Locus chr1:179126604
Transcript NM_001168239
WT Expression Level 5.27 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 10bp deletion in a coding exon of ABL2.
Description 10bp deletion
Parental Cell Line C631
Guide RNA Sequence AGTTCGCTCTAAGAATGGGC
PCR Primer Forward: ATCTGCAGTGGTATTGATCCTGTAG
Reverse: CTAGGTGAAAAGCTACGAGTCCTT
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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