A-Raf Antibody - CD BioSciences

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A-Raf Antibody

A-Raf Antibody

SPA-00106

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0.025 mL Online Inquiry
0.1 mL Online Inquiry
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Target Information
Target Name A-Raf
Gene Abbr. ARAF
Gene ID 369
Full Name A-Raf proto-oncogene, serine/threonine kinase
Alias A-RAF, ARAF1, PKS2, RAFA1
Introduction A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499. p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf. Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively. While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed. Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf. Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma. Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D2P9P
Immunogen Recombinant protein specific to a central region within human A-Raf protein.
Source/Purification Antibody is produced by immunizing animals with recombinant protein specific to a central region within human A-Raf protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:100)
MW(KDa) 68
Reactivity Human, Mouse, Rat
Sensitivity Endogenous
Specificity It recognizes endogenous levels of total A-Raf protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.