4E-BP1 Antibody - CD BioSciences

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4E-BP1 Antibody

4E-BP1 Antibody

SPA-00082

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
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Target Information
Target Name 4EBP
Gene Abbr. EIF4EBP1
Gene ID 1978
Full Name eukaryotic translation initiation factor 4E binding protein 1
Alias 4E-BP1, 4EBP1, BP-1, PHAS-I
Introduction Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen The immunogen recognized by this antibody maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978).
Usage
Application WB, IHC
Dilutions Western Blot (1:1000-1:6000); Immunohistochemistry (1:5000-1:20000)
MW(KDa) 15-20
Reactivity Human
Validation Knockout Validated.
Storage & Handling
Storage Buffer Tris-Citrate/Phosphate (pH 7.0-8.0).
Preservative 0.09% Sodium Azide
Storage Temp. Store at 4 °C.
Handling Do not freeze.

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