14-3-3 σ/Stratifin Antibody - CD BioSciences

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14-3-3 σ/Stratifin Antibody

14-3-3 σ/Stratifin Antibody

SPA-00018

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Target Information
Target Name 14-3-3
Gene Abbr. SFN
Gene ID 2810
Full Name stratifin
Alias YWHAS
Introduction The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively. Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions. 14-3-3 proteins may also induce target protein conformational changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.
Product Details
Host Goat
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen E. coli-derived recombinant human 14-3-3 sigma. Met1-Ser248. P31947.
Usage
Application WB
Dilutions Western Blot (1 µg/mL)
MW(KDa) 28
Reactivity Human, Mouse, Rat
Validation Knockout Validated.
Specificity Detects human, mouse, and rat 14-3-3 sigma in Western Blots. Does not cross react with other recombinant human 14‑3‑3 isoforms.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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