14-3-3 γ Antibody - CD BioSciences

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14-3-3 γ Antibody

14-3-3 γ Antibody

SPA-00046

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0.025 mL Online Inquiry
0.1 mL Online Inquiry
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Target Information
Target Name 14-3-3
Gene Abbr. YWHAG
Gene ID 7532
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
Alias 14-3-3GAMMA, DEE56, EIEE56, PPP1R170
Introduction The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively. Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions. 14-3-3 proteins may also induce target protein conformational changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. HS23
Isotype IgG1 Kappa
Immunogen N-terminal fragment of human 14-3-3 gamma [Uniprot: P61981].
Usage
Application WB, IF
Dilutions Western Blot (1:500-1:1000); Immunofluorescence (1:50-1:250)
MW(KDa) 27
Reactivity Human, Mouse, Rat, Bovine, Chicken, Zebrafish
Specificity It reacts with the human form of intact, non-modified N-terminus 14-3-3 gamma. The naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated is not recognized by this antibody.
Storage & Handling
Storage Buffer Ascites.
Preservative 0.1% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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