14-3-3 β/α Antibody - CD BioSciences

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14-3-3 β/α Antibody

14-3-3 β/α Antibody

SPA-00034

Size Price
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100 µg Online Inquiry
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Target Information
Target Name 14-3-3
Gene Abbr. YWHAB
Gene ID 7529
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
Alias GW128, HEL-S-1, HS1, KCIP-1, YWHAA
Introduction The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively. Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions. 14-3-3 proteins may also induce target protein conformational changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 40
Isotype IgG2B
Immunogen Recombinant rat 14-3-3 beta/alpha.
Usage
Application WB, IP
Dilutions Western Blot (1-5 µg/mL); Immunoprecipitation (5-10 µg/mL)
MW(KDa) 28
Reactivity Mouse, Rat
Storage & Handling
Storage Buffer PBS (pH 7.2), 10 mg/mL Sucrose and 1.0 mg/mL Sodium Azide.
Preservative Sodium Azide
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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