Quantitative Proteomics Service for Plant Methylation

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Quantitative Proteomics Service for Plant Methylation

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Methylation usually occurs on transcription factors and histones, especially on arginine (R) and lysine (K) residues. Arginine methylation can regulate RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation can regulate histone function and participate in epigenetic regulation of gene transcription.

CD BioSciences uses specific antibodies to enrich methylated peptide fragments and bind them to LC-MS/MS to enable quantitative analysis of large-scale methylated proteins. Our services help clients investigate the function of methylated proteins in regulating histone function and regulating the binding of proteins to other molecules in plants.

Protocol schematic for proteome-wide capture and identification of candidate methylated proteins using 3xMBT. Figure 1. Protocol schematic for proteome-wide capture and identification of candidate methylated proteins using 3xMBT. (Moore KE, et al. 2013)

Service Content Description
SILAC Service We investigated the dynamic changes of lysine-methylated and arginine-methylated proteins using antibody enrichment and SILAC labeling. We can compare the abundance of methylated peptides under different experimental conditions.
4D-Label Free Service Our 4D-Label Free service is based on the tims TOF Pro mass spectrometer and performs methylation differential proteomic analysis using ddaPASE scanning mode. Our service is applied to all samples, with less sample loading and faster scanning speed.

Technical Advantages

Professional testing and analysis capabilities.

Obtain consistent and reproducible measurement results.

High specificity and high throughput.

High resolution and sensitivity.

Notification for Sample Preparation and Handling
Plant leaves, wet weight ≥ 3g/sample.
Plant rhizome, xylem, phloem, etc., wet weight ≥ 5g/sample.
Cell samples, cells ≥ 5×107.
Protein extract, concentration > 2mg/mL, total amount ≥ 1mg, samples should not contain components that will affect the separation effect, such as nucleic acids, lipids, and polysaccharides.
Please use sufficient dry ice for transportation.

Application Fields

We have established advanced strategies for highly specific enrichment of lysine or arginine, with sensitive HPLC-MS/MS capable of analyzing various types of methylation. Our services can be applied to various fields of methylated protein analysis, such as

  • Chromatin structure and transcriptional regulation.
  • Stabilizing proteins, regulating protein transcription and binding activity.
  • Research on DNA replication and damage repair.
  • Epigenetic research.
  • Signal transduction studies on the immune process of plant disease resistance.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

Reference

  1. Moore KE, et al. (2013). A general molecular affinity strategy for global detection and proteomic analysis of lysine methylation. Mol Cell. 50(3):444-456.

For research use only, not for clinical use.