Plant Yeast Hybrid cDNA Library Construction Service
Prey Library Construction for Yeast Hybrid Screening
CD BioSciences provides plant yeast hybrid cDNA library construction services for downstream Y1H and Y2H screening. A well-constructed prey library is essential for recovering biologically meaningful candidate interactors.
The service can include plant sample evaluation, total RNA extraction, mRNA purification, cDNA synthesis, size selection, vector recombination, library transformation, QC analysis, plasmid preparation, and project reporting.
Service Overview
Yeast hybrid cDNA libraries represent expressed transcripts from selected plant tissues, treatments, or developmental stages. Library quality affects screening coverage, especially for low-abundance transcripts, tissue-specific interactors, and full-length coding sequences.
Figure 1. Workflow of plant yeast hybrid cDNA library construction.
Library Construction Strategies
| Strategy | Typical Use | Notes |
|---|---|---|
| SMART-based cDNA library | Projects with limited starting material or routine transcript representation needs. | Useful for efficient cDNA amplification and library construction. |
| Gateway or recombination-based library | Projects requiring vector transfer flexibility or recombination-compatible workflows. | Can support downstream transfer into different yeast hybrid vectors. |
| Three-frame library | Y2H prey libraries where reading-frame coverage is important. | Helps reduce frame-related loss of potential prey coding sequences. |
| Normalized library | Projects where low-abundance transcripts are important. | May reduce dominance of highly expressed transcripts, depending on project design. |
Quality Control Metrics
Library QC should be reported in a way that allows researchers to assess whether the library is suitable for screening. QC items may include RNA integrity, mRNA purification, ds-cDNA synthesis, insert distribution, library titer, positive clone rate, and library complexity.
Figure 2. Key QC metrics for plant yeast hybrid cDNA library construction.
Assessment of RNA purity, integrity, and suitability for cDNA synthesis.
PCR-based or electrophoretic evaluation of cDNA insert size range.
Estimation of viable transformants or colony-forming units in the constructed library.
Determination of the proportion of clones containing inserts.
Sample Requirements
- Plant tissue, cells, seedlings, or other project-specific material
- Species, tissue type, treatment condition, and collection time information
- Recommended biological diversity when the goal is broad interactor discovery
- Fresh, frozen, or preserved samples suitable for RNA extraction
- Existing total RNA or mRNA, if already prepared
- Target downstream application: Y1H, Y2H, nuclear Y2H, or membrane Y2H
Deliverables
- Library construction report
- RNA, mRNA, and ds-cDNA QC information
- Library titer and positive clone rate
- Insert size distribution data
- Library plasmid or bacterial library aliquot, when included in project scope
- Original images and experimental records
Physical library materials, backup storage, and reuse for future screening should be confirmed during project setup because deliverables may vary by library type and project agreement.
Related Services
Please contact us with your plant species, sample type, downstream screening system, and desired library coverage for project evaluation.
For research use only, not for clinical use.