Plant Gene Expression Level Assay Service
CD BioSciences has advanced equipment, extensive experience in molecular biology analysis, and a strict quality control system. We provide clients with plant gene expression level assay services based on real-time qPCR, Northern Blot, and microarray, laying the foundation for understanding gene function, researching biological processes, biomarker discovery, and crop improvement.
Gene expression level assay is an essential step in biological experiments. The expression of plant functional proteins requires the involvement of multiple steps from gene transcription to translation. Therefore, detailed detection of gene expression levels at each node can comprehensively understand the working principles of each biological system in plants.
Service Content
Real-time qPCR service
We provide RT-qPCR that can detect the total amount of product after each polymerase chain reaction cycle, providing real-time detection of the PCR process. Detailed services include
- Designing specific primers or fluorescent probes.
- Screening and validation of internal reference genes.
- Extraction of total RNA from plant samples.
- Plant miRNA sequence extension.
- Reverse transcription of plant RNA to cDNA.
- RT-qPCR standard curve plotting.
- Analysis of differences in plant gene expression.
Northern Blot service
We offer Northern Blot hybridization technology to detect the specific RNAs that can be used for quantitative or qualitative analysis of transcript expression.
Northern Blot experimental process
Microarray service
We offer microarray services that can simultaneously detect several tens of mRNA transcripts for gene expression or genomic DNA fragments for copy number variation analysis. Our services include but are not limited to
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Materials to be Provided by Clients
Plant cell count > 106
The total amount of plant tissue > 100mg
The total amount of plant DNA or RNA > 2μg
Target gene information, including gene sequence or GenBank accession number, etc.
Background information, including DNA/RNA source, gene abundance, etc.
Application Fields
- Researching gene regulatory networks, signaling pathways, and molecular mechanisms of plant development and growth.
- Identify genes related to stress tolerance, disease resistance, and specific developmental stages.
- Crop improvement, breeding programs, and monitoring plant health and quality.
- Understanding plant-microbe interactions to develop sustainable agricultural practices and biocontrol strategies.
CD BioSciences provides clients with comprehensive solutions to study the expression patterns of plant genes. With our advanced facilities and expertise in plant molecular biology, we provide cutting-edge services to elucidate the regulatory mechanisms and functional roles of plant genes. Please contact us if you need to examine the expression levels of plant genes. We are committed to providing reliable and insightful data to advance your research.
References
- Wei L, et al. (2013). Identification and testing of reference genes for Sesame gene expression analysis by quantitative real-time PCR. Planta. 237(3):873-889.
- Tirumalai V, et al. (2020). RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs. J Vis Exp. (161):10.3791/61394.
- Yang C, Wei H. (2015). Designing microarray and RNA-Seq experiments for greater systems biology discovery in modern plant genomics. Mol Plant. 8(2):196-206.
For research use only, not for clinical use.