V-PPase | vacuolar H+-pyrophosphatase
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V-PPase | vacuolar H+-pyrophosphatase

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Cat: PA00587
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Cucumis sativus, Dunaliella bardawil, Hordeum vulgare, Nicotiana tabacum, Picea abies, Vitis vinifera
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,UniProt P31414, TAIR AT1G15690
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 8000 (ELISA), 1 : 100 (IL), 1 : 2000 (WB)
Expected | apparent MW
80.8 | 73 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Cucumis sativus, Dunaliella bardawil, Hordeum vulgare, Nicotiana tabacum, Picea abies, Vitis vinifera
Predicted reactivity
Oryza sativa, Ricinus communis, Populus trichocarpa, Saccharum officinarum, Zea mays, Vigna radiata
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibodies will detect target protein in 1 µg of a crude membrane preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, less than µg load per well should be sufficient,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

Description

V-PPase(pyrophosphate-activated vacuolar membrane proton pump 1), EC=3.6.1.1, acts to establish a proton gradient, which is generally greater than the gradient of H+ -ATPase. Involved in auxin transport, NaCl and drought tolerance. Alternative names: pyrophosphate-activated inorganic pyrophosphatase 1,H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar proton pyrophosphatase 3

For research use only, not for clinical use.