V-ATPase | Epsilon subunit of tonoplast H+ATPase (goat antibody)
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V-ATPase | Epsilon subunit of tonoplast H+ATPase (goat antibody)

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Cat: PA00582
Size: 300 µl
Host: Goat
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
Host
Goat
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 300 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000-1 : 3000 (WB)
Expected | apparent MW
26 | 31 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum
Predicted reactivity
Algae, Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa,  Petunia sp.,Phaseolus sp. , Physcomitrium patens,  Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, Vitis vinifera      Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample. 2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Description

Plant vacuole V-ATPase is responsible for energy transport of ions and metabolites and acts as a "steward" and stress response enzyme. V-atpase is a multisubunit enzyme composed of membrane region and cytoplasmic catalytic region. It is related to FoF1 ATP synthase. Replacement protein name: vacuolar proton pump subunit E, protein Embryo defect 2448

For research use only, not for clinical use.