V-ATPase, a1 | vacuolar H+-ATPase subunit a isoform 1

Inquiry

Cat: PA00765

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A, UniProt: Q8RWZ7-1, TAIR: AT2G28520

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

93 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Capsella rubella, Cucumis sativus, Erythranthe guttata , Glycine soja, Lupinus angustifolius, Morus notabilis, Phaseolus vulgaris , Vitis vinifera

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Description

The V-ATPase subunit VHA-a1 is a subunit of the membrane integral Vo subunit. VHA-a1 targets V-ATPase to Arabidopsis trans-Golgi network/Earle endosomes (TGN/EE). This enzyme is involved in the acidification process of various parts of eukaryotic cells. This protein is encoded by the VHA-a1 gene AT2G28520. Nicknames: vacuole H(+) -ATPase a1 subunit, V-ATPase 93 kDa subunit.

For research use only, not for clinical use.