V-ATPase, a | Vacuolar H+ ATPase, subunit a

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar membranes are used, one µg load per well should be sufficient. Protocol of isolation of plant vacuolar membranes can be found here.,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

V-atpase subunit a is encoded by the VHA-A2 gene. It has hydrogen ion transmembrane transport activity. Alternative names :At2g21410/F3K23.17, putative vacuolar proton-ATPase subunit, V-ATPase subunit a (100 kDa subunit), VHA-a