V-ATPase, A | Vacuolar H+-ATPase subunit A (ammonium sulfate purified IgG)

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube label. Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. Protocol for isolation of plant vacuolar membranes can be found here.,Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

V-ATPase subunit A is the catalytic subunit of the vacuolar ATPase V1 complex. This enzyme (EC=3.6.3.14) is involved in the acidification process of various components of eukaryotic cells. This protein is encoded by the VHA-A gene. Nicknames: vacuole proton pump alpha subunit, vacuole H(+) -ATPase A subunit, V-ATPase 69kda subunit