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UDP-glycosyltransferase 75B1 (UGT75B1), Recombinant Protein
Cat: RP07994
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 75B1 (UGT75B1)
Product Gene Name
UGT75B1 recombinant protein
Product Synonym Gene Name
UGT75B1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MAPPHFLLVT FPAQGHVNPS LRFARRLIKR TGARVTFVTC VSVFHNSMIA NHNKVENLSF LTFSDGFDDG GISTYEDRQK RSVNLKVNGD KALSDFIEAT KNGDSPVTCL IYTILLNWAP KVARRFQLPS ALLWIQPALV FNIYYTHFMG NKSVFELPNL SSLEIRDLPS FLTPSNTNKG AYDAFQEMME FLIKETKPKI LINTFDSLEP EALTAFPNID MVAVGPLLPT EIFSGSTNKS VKDQSSSYTL WLDSKTESSV IYVSFGTMVE LSKKQIEELA RALIEGKRPF LWVITDKSNR ETKTEGEEET EIEKIAGFRH ELEEVGMIVS WCSQIEVLSH RAVGCFVTHC GWSSTLESLV LGVPVVAFPM WSDQPTNAKL LEESWKTGVR VRENKDGLVE RGEIRRCLEA VMEEKSVELR ENAKKWKRLA MEAGREGGSS DKNMEAFVED ICGESLIQNL CEAEEVKVK
Sequence Positions
1-469, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
52,813 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
UDP-glycosyltransferase
NCBI/Uniprot Data
NCBI Accession #
NP_563742.1
NCBI GI #
18390540
NCBI GenBank Nucleotide #
NM_100435.3
NCBI GeneID
837058
NCBI Official Full Name
UDP-glucosyltransferase 75B1
NCBI Official Symbol
UGT75B1
NCBI Official Synonym Symbols
T25N20.21; UDP-GLUCOSE TRANSFERASE 1; UDP-GLUCOSYLTRANSFERASE; UDP-glucosyltransferase 75B1; UGT1
NCBI Protein Information
UDP-glucosyltransferase 75B1
NCBI Summary
A UDP-glucose transferase localized in the phragmoplast. It has been co-purified with the callose synthase complex and may transfer UDP-glucose from sucrose synthase to the callose synthase and thus help form a substrate channel for the synthesis of callose at the forming cell plate. Induced by salicylic acid. Independent of NPR1 for their induction by salicylic acid. UGT1 encodes a protein with glucosyltransferase activity with high sequence homology to UGT2 (AT1G05530). It belongs to an UGT subfamily that binds UDP-glucose but not UDP-glucuronate, UDP-galactose, or UDP-rhamnose as the glycosyl donor. UGT1 was shown to be able to use abscisic acid as glycosylation substrate in the presence of UDP-glucose. UGT1/UGT75B1 catalyzes the formation of the p-aminobenzoate-glucose ester in vitro and in vivo. It appears to be the enzyme predominantly responsible for pABA-Glc formation in Arabidopsis based on assays in leaves, flowers, and siliques.
UniProt Gene Name
UGT75B1
UniProt Synonym Gene Names
UGT1
UniProt Protein Name
UDP-glycosyltransferase 75B1
UniProt Synonym Protein Names
(Uridine 5'-diphosphate-glucose:indol-3-ylacetyl)-beta-D-glucosyl transferase 1; IAA-Glu synthase 1; Indole-3-acetate beta-glucosyltransferase 1 (EC:2.4.1.121)
UniProt Primary Accession #
Q9LR44
UniProt Related Accession #
Q9LR44
UniProt Comments
Possesses low catalytic activity on indole-3-acetic acid (IAA) in vitro. May transfer UDP-glucose from sucrose synthase to callose synthase for the synthesis of callose at the forming cell plate during cytokinesis. Has high affinity for 4-aminobenzoate. Catalyzes the formation of 4-aminobenzoate glucose ester which represents a storage form of 4-aminobenzoate in the vacuole. Is the major source of this activity in the plant. Also active in vitro on benzoates and benzoate derivatives.
For research use only, not for clinical use.