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Ubiquitin-conjugating enzyme E2 variant 1B (UEV1B), Recombinant Protein
Cat: RP04028
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 variant 1B (UEV1B)
Product Gene Name
UEV1B recombinant protein
Product Synonym Gene Name
UEV1B
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MGSEEEKVVV PRNFRLLEEL ERGEKGIGDG TVSYGMDDAD DILMQSWTGT ILGPHNTAYE GKIFQLKLFC GKDYPESPPT VRFQSRINMA CVNPENGVVD PSHFPMLSNW RREFTMEDLL IQLKKEMMSS QNRKLAQPLE GNEEGRTDPK GLVVKCCVM
Sequence Positions
1-159, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
18,001 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Ubiquitin-conjugating enzyme
NCBI/Uniprot Data
NCBI Accession #
NP_564994.1
NCBI GI #
18409647
NCBI GenBank Nucleotide #
NM_105734.3
NCBI GeneID
843403
NCBI Official Full Name
MMS ZWEI homologue 2
NCBI Official Symbol
MMZ2
NCBI Official Synonym Symbols
F5A18.16; F5A18_16; MMS ZWEI homologue 2; UBIQUITIN E2 VARIANT 1B; UEV1B
NCBI Protein Information
MMS ZWEI homologue 2
NCBI Summary
MMZ2/UEV1B encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UEV1A can form diubiquitin and triubiquitin chains in combination with UBC13A/UBC35 in vitro. It can also functionally complement an mms2 mutation in budding yeast, both by increasing mms2 mutant viability in the presence of the DNA damaging agent MMS, and by reducing the rate of spontaneous DNA mutation. However, a combination of MMZ2/UEV1B and UBC13A do not do a good job of rescuing an mms2 ubc13 double mutant in yeast. MMZ2/UEV1B transcripts are found in most plant organs, but not in the pollen or in seedlings 6 hours or 2 days post-germination. The transcript levels do not appear to be stress-inducible.
UniProt Gene Name
UEV1B
UniProt Synonym Gene Names
MMZ2; Ubc enzyme variant 1B
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 variant 1B
UniProt Synonym Protein Names
Protein MMS ZWEI HOMOLOG 2
UniProt Primary Accession #
Q9CAB6
UniProt Secondary Accession #
Q8L9N0
UniProt Related Accession #
Q9CAB6
UniProt Comments
Has no ubiquitin ligase activity on its own. The heterodimer with UBC catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. May play a role in the control of progress through the cell cycle and differentiation. May play a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
For research use only, not for clinical use.