Ubiquitin-conjugating enzyme E2 35 (UBC35), Recombinant Protein
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Ubiquitin-conjugating enzyme E2 35 (UBC35), Recombinant Protein

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Cat: RP04783
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 35 (UBC35)
Product Gene Name
UBC35 recombinant protein
Product Synonym Gene Name
UBC35
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MANSNLPRRI IKETQRLLSE PAPGISASPS EDNMRYFNVM ILGPTQSPYE GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVD TAKEWTRLYA SGA
Sequence Positions
1-153, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
12,851 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Ubiquitin-conjugating enzyme

NCBI/Uniprot Data

NCBI Accession #
NP_001031298.1
NCBI GI #
79321461
NCBI GenBank Nucleotide #
NM_001036221.3
NCBI GeneID
844224
NCBI Official Full Name
ubiquitin-conjugating enzyme 35
NCBI Official Symbol
UBC35
NCBI Official Synonym Symbols
F9K20.8; F9K20_8; UBC13A; UBIQUITIN CONJUGATING ENZYME 13A; ubiquitin-conjugating enzyme 35
NCBI Protein Information
ubiquitin-conjugating enzyme 35
NCBI Summary
UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutant's viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B.
UniProt Gene Name
UBC35
UniProt Synonym Gene Names
UBC13A; UBG13A
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 35
UniProt Synonym Protein Names
E2 ubiquitin-conjugating enzyme 35; Ubiquitin carrier protein 35
UniProt Primary Accession #
Q94A97
UniProt Secondary Accession #
Q2V4C1; Q9ZVA6
UniProt Related Accession #
Q94A97
UniProt Comments
Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.

For research use only, not for clinical use.