Tubulin alpha chain (polyclonal antibodies)

Cat: PA00448

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardii, Euglena gracilis, Hordeum vulgare, Oryza sativa, Setaria italica, Zea mays

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from available tubulin alpha chain sequences including Arabidopsis thaliana tubulin alpha-1-chain P11139(At1g64740), alpha-2/alpha-4 chain B9DGT7(At1g50010), alpha-5 chain B9DHQ0(At5g19780), alpha-6-chain P29511(At4g14960)Peptide used to elict this antibody is not present in tubulin beta.

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Western blot (WB)

Recommended dilution

1 : 500 (IF), 1 : 1000 (WB)

Expected | apparent MW

49 | 52 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardii, Euglena gracilis, Hordeum vulgare, Oryza sativa, Setaria italica, Zea mays

Predicted reactivity

Brassica napus, Chlorella vulgaris, Chlorella variabilis, Cucumis sativus, Euglena gracilis, Glycine max, Micromonoas pusilla, Nannochloropsis gaditana, Ostreococcus lucimarinus, Pisum sativum, Physcomitrium patens, Picea sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Triticum aestivum, Vigna radiata, Vitis vinifera

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here.

Description

Microtubulin (TUA) and tubulin together form microtubules.

For research use only, not for clinical use.