Transcription factor PIF1 (PIF1), Recombinant Protein
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Transcription factor PIF1 (PIF1), Recombinant Protein

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Cat: RP02548
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Transcription factor PIF1 (PIF1)
Product Gene Name
PIF1 recombinant protein
Product Synonym Gene Name
PIF1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MHHFVPDFDT DDDYVNNHNS SLNHLPRKSI TTMGEDDDLM ELLWQNGQVV VQNQRLHTKK PSSSPPKLLP SMDPQQQPSS DQNLFIQEDE MTSWLHYPLR DDDFCSDLLF SAAPTATATA TVSQVTAARP PVSSTNESRP PVRNFMNFSR LRGDFNNGRG GESGPLLSKA VVRESTQVSP SATPSAAASE SGLTRRTDGT DSSAVAGGGA YNRKGKAVAM TAPAIEITGT SSSVVSKSEI EPEKTNVDDR KRKEREATTT DETESRSEET KQARVSTTST KRSRAAEVHN LSERKRRDRI NERMKALQEL IPRCNKSDKA SMLDEAIEYM KSLQLQIQMM SMGCGMMPMM YPGMQQYMPH MAMGMGMNQP IPPPSFMPFP NMLAAQRPLP TQTHMAGSGP QYPVHASDPS RVFVPNQQYD PTSGQPQYPA GYTDPYQQFR GLHPTQPPQF QNQATSYPSS SRVSSSKESE DHGNHTTG
Sequence Positions
1-478, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
44,715 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Transcription factor

NCBI/Uniprot Data

NCBI Accession #
NP_001189559.1
NCBI GI #
334184322
NCBI GenBank Nucleotide #
NM_001202630.2
NCBI GeneID
816538
NCBI Official Full Name
phytochrome interacting factor 3-like 5
NCBI Official Symbol
PIL5
NCBI Official Synonym Symbols
PHY-INTERACTING FACTOR 1; phytochrome interacting factor 3-like 5; PIF1; T2G17.2; T2G17_2
NCBI Protein Information
phytochrome interacting factor 3-like 5
NCBI Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
UniProt Gene Name
PIF1
UniProt Synonym Gene Names
BHLH15; EN101; PIL5; AtbHLH15; bHLH 15
UniProt Protein Name
Transcription factor PIF1
UniProt Synonym Protein Names
Basic helix-loop-helix protein 15; AtbHLH15; bHLH 15; Protein PHY-INTERACTING FACTOR 1; Protein PHYTOCHROME INTERACTING FACTOR 3-LIKE 5; Transcription factor EN 101; bHLH transcription factor bHLH015
UniProt Primary Accession #
Q8GZM7
UniProt Secondary Accession #
Q0WQ83; Q3EBY0; Q9SL63
UniProt Related Accession #
Q8GZM7
UniProt Comments
Transcription activator. Regulates negatively chlorophyll biosynthesis and seed germination in the dark, and lightinduced degradation of PIF1 relieves this negative regulation to promote photomorphogenesis. Binds to the G-box motif (5'-CACGTG-3') found in many light-regulated promoters. Promotes the expression of SOM, and thus modulates responses to abscisic acid (ABA) and gibberellic acid (GA).

For research use only, not for clinical use.