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Transcription factor MYB12 (MYB12), Recombinant Protein
Cat: RP04013
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Transcription factor MYB12 (MYB12)
Product Gene Name
MYB12 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MGRAPCCEKV GIKRGRWTAE EDQILSNYIQ SNGEGSWRSL PKNAGLKRCG KSCRLRWINY LRSDLKRGNI TPEEEELVVK LHSTLGNRWS LIAGHLPGRT DNEIKNYWNS HLSRKLHNFI RKPSISQDVS AVIMTNASSA PPPPQAKRRL GRTSRSAMKP KIHRTKTRKT KKTSAPPEPN ADVAGADKEA LMVESSGAEA ELGRPCDYYG DDCNKNLMSI NGDNGVLTFD DDIIDLLLDE SDPGHLYTNT TCGGDGELHN IRDSEGARGF SDTWNQGNLD CLLQSCPSVE SFLNYDHQVN DASTDEFIDW DCVWQEGSDN NLWHEKENPD SMVSWLLDGD DEATIGNSNC ENFGEPLDHD DESALVAWLL S
Sequence Positions
1-371, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
41,394 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Transcription factor
NCBI/Uniprot Data
NCBI Accession #
NP_182268.1
NCBI GI #
15226609
NCBI GenBank Nucleotide #
NM_130314.4
NCBI GeneID
819359
NCBI Official Full Name
myb domain protein 12
NCBI Official Symbol
MYB12
NCBI Official Synonym Symbols
ATMYB12; myb domain protein 12; MYB DOMAIN PROTEIN 12; PFG1; PRODUCTION OF FLAVONOL GLYCOSIDES 1; T30B22.24
NCBI Protein Information
myb domain protein 12
NCBI Summary
''MYB12 belongs to subgroup 7 of the R2R3-MYB family. It strongly activates the promoters of chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and - to a lesser extent - chalcone flavanone isomerase (CHI), but cannot activate the promoters of flavonoid-3'hydroxylase (F3'H) and dihydroflavonol 4-reductase (DF). The activation requires a functional MYB recognition element (MRE). Results from the myb12-1f allele indicate that an activation domain might be present in the C-terminus. Overexpression or knock-out plants do not show any obvious phenotype under greenhouse conditions. Young myb12-ko seedlings contain reduced amounts of flavonoids (quercetin and kaempferol), while seedlings as well as leaves of MYB12-OX plants displayed an increased flavonoid content. They did not show any significant difference in anthocyanin content. Expression of CHS and FLS shows a clear correlation to MYB12 expression levels. CHI and F3H show increased transcript levels in the MYB12-OX lines, but no differences in the knock-out. Even in the absence of functional MYB12, flavonol biosynthesis is not completely absent, suggesting functional redundancy. '' The redundant factors are MYB11 and MYB111 although MYB12 is primarily required for flavonol biosynthesis in roots. Mutations in MYB12 block both auxin and ethylene stimulation of flavonoid synthesis.
UniProt Gene Name
MYB12
UniProt Synonym Gene Names
AtMYB12
UniProt Protein Name
Transcription factor MYB12
UniProt Synonym Protein Names
Myb-related protein 12
UniProt Primary Accession #
O22264
UniProt Secondary Accession #
Q38IY2; Q9SBG6
UniProt Related Accession #
O22264
UniProt Comments
Flavonol-specific transcription activator involved in the regulation of several genes of flavonoid biosynthesis. Activates the expression of CHS, CHI, F3H and FLS1. Controls flavonol biosynthesis mainly in the root (PubMed:17419845, PubMed:20731781). Confers tolerance to UV-B (PubMed:19895401).
For research use only, not for clinical use.