TOC1-P | TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated
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TOC1-P | TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated

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Cat: PA01274
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana TOC1 phosphorylated protein sequence, UniProt: A0A178UC73, TAIR: AT5G61380
Host
Rabbit
Clonality
Polyclonal
Purity
Antigen affinity purified serum, in PBS pH 7.4
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile or deionized water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Application
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
69.195 | kDa (due to N-terminal or C-terminal processing)

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

TOC1 is heat sensitive and requires specific extraction buffer and denaturation conditions, described in application example. Using other conditions, may contribute to lack of detection of phosphorylation of TOC1 using this antibody.

Description

Sequence of TOC1 / CAB expression 1) involved in the negative regulation of gene expression and cytokinin activation signal. Influence carbon fixation and biomass through circadian cycles.

For research use only, not for clinical use.