Immunogen
KLH-conjugated peptide containing phospho-Thr, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins: S6K1 and S6K2.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Never Store this antibody in 4°C
Application
Western blot (WB)
Recommended dilution
1 : 500 (WB)
Expected | apparent MW
52,6 kDa (S6K1) and 53 kDa (S6K2) | 60 kDa