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ROS1 (ROS1), Recombinant Protein
Cat: RP03680
Size: 0.02 mg/ 0.1 mg/ 1 mg/ 5x1 mg
Species: Arabidopsis halleri
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Protein ROS1 (ROS1), partial
Product Synonym Names
DEMETER-like protein 1
Product Gene Name
ROS1 recombinant protein
Purity
Greater than 90% as determined by SDS-PAGE.
Sequence Positions
Partial, 1-320aa
Format
Tris-based buffer, 50% glycerol
Host
E Coli
Molecular Weight
52.3 kDa
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Protein Family
Protein
NCBI/Uniprot Data
NCBI Accession #
NP_181190.3
NCBI GI #
42569673
NCBI GenBank Nucleotide #
NM_129207.5
NCBI GeneID
818224
NCBI Official Full Name
demeter-like 1
NCBI Official Symbol
DML1
NCBI Official Synonym Symbols
AtROS1; demeter-like 1; F1O11.12; F1O11_12; REPRESSOR OF SILENCING1; ROS1
NCBI Protein Information
demeter-like 1
NCBI Summary
A repressor of transcriptional gene silencing. Functions by demethylating the target promoter DNA. Interacts physically with RPA2/ROR1. In the ros1 mutants, an increase in methylation is observed in a number of gene promoters. Among the loci affected by ros1, a few (RD29A and At1g76930) are affected in cytosine methylation in all sequence contexts (CpG, CpNpG or CpNpN), although many others are affected primarily in non-CpG contexts.
UniProt Gene Name
ROS1
UniProt Synonym Gene Names
DML1
UniProt Protein Name
Protein ROS1
UniProt Synonym Protein Names
DEMETER-like protein 1; Repressor of silencing 1
UniProt Primary Accession #
Q9SJQ6
UniProt Secondary Accession #
Q7Y1X6
UniProt Related Accession #
Q9SJQ6
UniProt Comments
Bifunctional DNA glycosylase/lyase, which excises 5-methylcytosine (5-meC) and 5-hydroxymethylcytosine (5-hmeC), leaving an apyrimidinic (AP) site that is subsequently incised by the lyase activity (PubMed:25240767). Generates 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) as a primary 5-meC excision intermediate (PubMed:25228464). Prevents DNA hypermethylation, specifically in the promoter of otherwise silenced loci. May be involved in DNA repair through its nicking activity on methylated DNA. Binds with similar affinity to both methylated and non-methylated DNA. Highly distributive behavior on DNA substrates containing multiple 5-meC residues. Involved with Pol IV in the remodeling of the 5S rDNA chromatin via DNA methylation modifications during the first days of development post-germination. Participates in UV-B induced- and oxidative DNA damage repair (PubMed:24155752).
For research use only, not for clinical use.