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RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1), Recombinant Protein
Cat: RP02575
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1) , partial
Product Gene Name
CPL1 recombinant protein
Product Synonym Gene Name
CPL1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
108,421 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
RNA polymerase II C-terminal domain phosphatase
NCBI/Uniprot Data
NCBI Accession #
NP_193898.3
NCBI GI #
79481163
NCBI GenBank Nucleotide #
NM_118287.5
NCBI GeneID
828254
NCBI Official Full Name
C-terminal domain phosphatase-like 1
NCBI Official Symbol
CPL1
NCBI Official Synonym Symbols
ATCPL1; ATCPL2; C-terminal domain phosphatase-like 1; C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1; F17L22.130; F17L22_130; FIERY 2; FRY2; RCF2; regulators of C-REPEAT BINDING FACTOR (CBF) gene expression 2; SHI4; SHINY 4
NCBI Protein Information
C-terminal domain phosphatase-like 1
NCBI Summary
encodes a a novel transcriptional repressor harboring two double-stranded RNA-binding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. Protein exhibits innate phosphatase activity in vitro. Mutants exhibit hyperresponsiveness to ABA, cold, and NaCl.
UniProt Gene Name
CPL1
UniProt Synonym Gene Names
CTD phosphatase-like 1
UniProt Protein Name
RNA polymerase II C-terminal domain phosphatase-like 1
UniProt Synonym Protein Names
Carboxyl-terminal phosphatase-like 1; AtCPL1
UniProt Primary Accession #
Q5YDB6
UniProt Secondary Accession #
Q0WNZ7; Q56WT8; Q9SVT0
UniProt Related Accession #
Q5YDB6
UniProt Comments
Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL2, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated and jasmonic acid (JA) mediated signaling pathways, NaCl, osmotic stress, wounding, and cold resistance. Regulates negatively the expression of jasmonic acid (JA) biosynthetic genes in response to wounding (PubMed:11874572, PubMed:12149434, PubMed:12149453, PubMed:15388846, PubMed:18506580, PubMed:18764923). Forms a complex with RCF3 that modulates co-transcriptional processes such as mRNA capping and polyadenylation, and functions to repress stress-inducible gene expression (PubMed:23874224). Dephosphorylates RCF3 (PubMed:26227967). Involved in the dephosphorylation of EIF4A3. This dephosphorylation retains EIF4A3 in the nucleus and limits its accumulation in the cytoplasm. Is essential for the degradation of the nonsense-mediated mRNA decay (NMD) transcripts (PubMed:26887918).
For research use only, not for clinical use.