Putative quinone-oxidoreductase homolog, chloroplastic (At4g13010), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Putative quinone-oxidoreductase homolog, chloroplastic (At4g13010)

Product Gene Name

At4g13010 recombinant protein

Product Synonym Gene Name

At4g13010

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MAGKLMHALQ YNSYGGGAAG LEHVQVPVPT PKSNEVCLKL EATSLNPVDW KIQKGMIRPF LPRKFPCIPA TDVAGEVVEV GSGVKNFKAG DKVVAVLSHL GGGGLAEFAV ATEKLTVKRP QEVGAAEAAA LPVAGLTALQ ALTNPAGLKL DGTGKKANIL VTAASGGVGH YAVQLAKLAN AHVTATCGAR NIEFVKSLGA DEVLDYKTPE GAALKSPSGK KYDAVVHCAN GIPFSVFEPN LSENGKVIDI TPGPNAMWTY AVKKITMSKK QLVPLLLIPK AENLEFMVNL VKEGKVKTVI DSKHPLSKAE DAWAKSIDGH ATGKIIVEP

Sequence Positions

1-329, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

34,436 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Putative quinone-oxidoreductase

NCBI Accession #

NP_193037.1

NCBI GI #

15235549

NCBI GenBank Nucleotide #

NM_117370.3

NCBI GeneID

826914

NCBI Official Full Name

Oxidoreductase, zinc-binding dehydrogenase family protein

NCBI Official Symbol

AT4G13010

NCBI Official Synonym Symbols

F25G13.100; F25G13_100

NCBI Protein Information

Oxidoreductase, zinc-binding dehydrogenase family protein

UniProt Gene Name

CEQORH

UniProt Synonym Gene Names

ceQORH

UniProt Protein Name

Chloroplast envelope quinone oxidoreductase homolog

UniProt Primary Accession #

Q9SV68

UniProt Comments

NADPH-dependent alpha,beta-unsaturated oxoene reductase reducing the double bond of medium-chain (C9) to long-chain (C18) reactive electrophile species deriving from poly-unsaturated fatty acid peroxides. The best substrates are 13-lipoxygenase-derived gamma-ketols, but is unable to reduce the double bond of short-chain alkenals and alkenones such as acrolein, crotonaldehyde, 3-buten-2-one, 4-hexen-3-one and trans-2-hexenal, or quinones such as duroquinone, decylubiquinone, coenzyme Q0, menadione, menaquinone and phylloquinone. Can use trans-2-nonenal, trans-3-decen-2-one, 4-hydroxynonenal, 12-oxo-10(E) dodecanoate (traumatin), 4-oxononenal, trans-1,3 diphenyl-2-propenone, trans-1,4-diphenyl-2-butene-1,4-dione, 9-oxo-12,13-epoxy-(10E)-octadecenoic acid (trans-EKODE-1b), 9-hydroxy-12-oxo-10(E)-octadecenoic acid, 9-Hydroxy-12-oxo-10(E),15(Z)-octadecadienoic acid and 9,13-dihydroxy-10-oxo-11-octadecenoic acid as substrates, but has no activity with 13(R,S)-hydroperoxy-9(Z),11(E)-octadecadienoic acid (13-HPOD), 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid, 13-hydroxy-12-oxo-9(Z)-octadecenoic acid, 9-oxo-10(E),12(Z)-octadecadienoic acid (9-KODE), 13-oxo-9(Z),11(E)-octadecadienoic acid (13-KODE) and 12-oxo-10,15(Z)-phytodienoic acid (12-OPDA).