Protein mago nashi homolog (Os12g0287200, LOC_Os12g18880), Recombinant Protein

Full Product Name

Recombinant Oryza sativa subsp. japonica Protein mago nashi homolog (Os12g0287200, LOC_Os12g18880)

Product Gene Name

Os12g0287200 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MATGGAAGED VPGGGEFYLR YYVGHKGKFG HEFLEFEFRP DGKLRYANNS NYKNDTMIRK EVFVSPSVLR EARRIIQESE IMKEDDNNWP EPDRVGRQEL EIVMGNEHIS FTTSKIGSLV DVQTSKDPEG LRIFYYLVQD LKCFVFSLIN LHFKIKPIQS

Sequence Positions

1-160, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

18,430 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI Accession #

XP_015618267.1

NCBI GI #

1002309797

NCBI GenBank Nucleotide #

XM_015762781.1

NCBI GeneID

4351998

NCBI Official Full Name

protein mago nashi homolog

NCBI Official Symbol

LOC4351998

NCBI Protein Information

protein mago nashi homolog

UniProt Gene Name

MAGO2

UniProt Synonym Gene Names

OsMAGO2

UniProt Protein Name

Protein mago nashi homolog 2

UniProt Synonym Protein Names

Mago nashi-like protein 2Curated

UniProt Primary Accession #

P49030

UniProt Secondary Accession #

Q0INV7; Q2QTQ3; Q9AXD9

UniProt Related Accession #

P49030

UniProt Comments

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGO-Y14 heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGO-Y14 heterodimer interacts with the EJC key regulator PYM leading to EJC disassembly in the cytoplasm . EJC core heterodimers play essential roles in plant growth and development, and pollen and seed development (PubMed:25230811, PubMed:24820023). The MAGO-Y14 heterodimer selectively binds to the UDT1 (UNDEVELOPED TAPETUM 1) pre-mRNA transcript and regulates the splicing of UDT1, a key regulator in stamen development (PubMed:24820023).