Protein mago nashi homolog (At1g02140), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Protein mago nashi homolog (At1g02140)

Product Gene Name

At1g02140 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MAAEEATEFY LRYYVGHKGK FGHEFLEFEF REDGKLRYAN NSNYKNDTII RKEVFLTPAV LKECKRIVSE SEILKEDDNN WPEPDRVGKQ ELEIVLGNEH ISFATSKIGS LVDCQSSNDP EGLRIFYYLV QDLKCLVFSL ISLHFKIKPI

Sequence Positions

1-150, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

17,458 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI Accession #

NP_171716.1

NCBI GI #

15217623

NCBI GenBank Nucleotide #

NM_100094.3

NCBI GeneID

837560

NCBI Official Full Name

mago nashi family protein

NCBI Official Symbol

MAGO

NCBI Official Synonym Symbols

HAP1; HAPLESS 1; MAGO NASHI; MATERNAL EFFECT EMBRYO ARREST 63; MEE63; T7I23.7; T7I23_7

NCBI Protein Information

mago nashi family protein

NCBI Summary

Encodes a homologue of the exon junction complex (EJC) component MAGO that participates in intron-mediated enhancement of gene expression.

UniProt Gene Name

MAGO

UniProt Synonym Gene Names

AtMAGO

UniProt Protein Name

Protein mago nashi homolog

UniProt Primary Accession #

O23676

UniProt Secondary Accession #

A0A023T774

UniProt Related Accession #

O23676

UniProt Comments

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGO-Y14 heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGO-Y14 heterodimer interacts with the EJC key regulator PYM leading to EJC disassembly in the cytoplasm . Can increase in vitro the expression from reporter constructs that contain leader introns required for the expression of different genes. In association with MAGO and PYM, participates in intron-mediated enhancement of gene expression (PubMed:21676911). The MAGO-Y14 heterodimer works synergistically with the NMD pathway to regulate male gametophyte development (PubMed:26867216).