Products
Online Inquiry
Protein argonaute 9 (AGO9), Recombinant Protein
Cat: RP02631
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Protein argonaute 9 (AGO9) , partial
Product Gene Name
AGO9 recombinant protein
Product Synonym Gene Name
AGO9
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
100,524 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Protein argonaute
NCBI/Uniprot Data
NCBI Accession #
NP_197613.2
NCBI GI #
42568007
NCBI GenBank Nucleotide #
NM_122122.3
NCBI GeneID
832241
NCBI Official Full Name
Argonaute family protein
NCBI Official Symbol
AGO9
NCBI Official Synonym Symbols
ARGONAUTE 9; T10F18.180; T10F18_180
NCBI Protein Information
Argonaute family protein
NCBI Summary
AGO9-dependent sRNA silencing is crucial to specify cell fate in the Arabidopsis ovule. AGO9 is expressed in reproductive companion cells but not in the associated male or female gametes or their precursors. Therefore, AGO9 acts non-cell autonomously to silencing the activity of TEs activity in the female gametophyte.
UniProt Gene Name
AGO9
UniProt Protein Name
Protein argonaute 9
UniProt Primary Accession #
Q84VQ0
UniProt Secondary Accession #
Q56X15; Q84YI4
UniProt Related Accession #
Q84VQ0
UniProt Comments
Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Associates preferentially with small RNAs of 24 nucleotide in length with a 5' terminal adenosine. Interacts with 24 nucleotide sRNAs derived from transposable elements (TEs). Required to silence pericentrometric-located TEs in female gametes and their accessory cells. Necessary to inactivate a significant proportion of long terminal repeat retrotransposons (LTRs) in the ovule. Required to specify cell fate in ovule. Involved in the control of female gamete formation by restricting the specification of gametophyte precursors in a dosage-dependent, non-cell-autonomous manner. Targeted by turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO9 function in RNA silencing.
For research use only, not for clinical use.