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Protein argonaute 10 (AGO10), Recombinant Protein
Cat: RP05432
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Protein argonaute 10 (AGO10) , partial
Product Gene Name
AGO10 recombinant protein
Product Synonym Gene Name
AGO10
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
110,868 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Protein argonaute
NCBI/Uniprot Data
NCBI Accession #
NP_001190464.1
NCBI GI #
334188178
NCBI GenBank Nucleotide #
NM_001203535.1
NCBI GeneID
834403
NCBI Official Full Name
Stabilizer of iron transporter SufD / Polynucleotidyl transferase
NCBI Official Symbol
AGO10
NCBI Official Synonym Symbols
ARGONAUTE 10; ARGONAUTE PROTEIN 10; MQD19.17; MQD19_17; PINHEAD; PNH; ZLL; ZWILLE
NCBI Protein Information
Stabilizer of iron transporter SufD / Polynucleotidyl transferase
NCBI Summary
Encodes Argonaute10, a member of the EIF2C (elongation initiation factor 2c)/ Argonaute class of proteins. Required to establish the central-peripheral organization of the embryo apex. Along with WUS and CLV genes, controls the relative organization of central zone and peripheral zone cells in meristems. Acts in embryonic provascular tissue potentiating WUSCHEL function during meristem development in the embryo. AGO10 specifically sequesters miR166/165 to regulate shoot apical meristem development.
UniProt Gene Name
AGO10
UniProt Synonym Gene Names
PNH; ZLL
UniProt Protein Name
Protein argonaute 10
UniProt Synonym Protein Names
Protein PINHEAD; Protein ZWILLE
UniProt Primary Accession #
Q9XGW1
UniProt Secondary Accession #
O49256
UniProt Related Accession #
Q9XGW1
UniProt Comments
Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Required for reliable formation of primary and axillary shoot apical meristems. Specifies leaf adaxial identity by repressing the miR165 and miR166 microRNAs in the embryonic shoot apex, in the shoot apical meristem (SAM) and leaf. Represses the microRNA miR398 which targets CCS1 chaperone mRNAs for translational inhibition. Acts as a negative regulator of AGO1 protein level. Like AGO1, is required for stem cell function and organ polarity. Unlike AGO1, is not subjected to small RNA-mediated repression itself. Essential for multiple processes in development.
For research use only, not for clinical use.