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Probable histone H2AXa (At1g08880), Recombinant Protein
Cat: RP09233
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Probable histone H2AXa (At1g08880)
Product Gene Name
At1g08880 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MSTGAGSGTT KGGRGKPKAT KSVSRSSKAG LQFPVGRIAR FLKSGKYAER VGAGAPVYLS AVLEYLAAEV LELAGNAARD NKKTRIVPRH IQLAVRNDEE LSKLLGSVTI ANGGVLPNIH QTLLPSKVGK NKGDIGSASQ EF
Sequence Positions
1-142, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
14,813 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
NCBI/Uniprot Data
NCBI Accession #
NP_172363.1
NCBI GI #
15223948
NCBI GenBank Nucleotide #
NM_100760.5
NCBI GeneID
837409
NCBI Official Full Name
Histone superfamily protein
NCBI Official Symbol
H2AXA
NCBI Official Synonym Symbols
F7G19.24; F7G19_24; G-H2AX; GAMMA H2AX; gamma histone variant H2AX; GAMMA-H2AX; histone H2A 5; HTA5
NCBI Protein Information
Histone superfamily protein
NCBI Summary
Encodes HTA5, a histone H2A protein. H2AX is a meiosis-specific isoform of histone H2A. Upon DSB formation, rapid accumulation of phosphorylated H2AX (gamma-H2AX) occurs around the break site. #H2AX foci accumulate in early G2. Immunolocalization studies in spread preparations of wild-type meiocytes at G2/early leptotene revealed the accumulation of numerous rather diffuse gamma-H2AX foci throughout the chromatin. However, their accumulation is not contemporaneous with that of AtSPO11-1. At 3 h post-S, no gamma-H2AX foci are detected. During the 3- to 5-h window when AtSPO11-1 foci rapidly disappear, there is an equally swift accumulation of gamma-H2AX to a maximum of >50 diffuse foci. The level of gammaH2AX then remains constant for a further 13 h before undergoing a gradual decrease to 10#Aei20 foci in the 18- to 24-h post-S period. By 30 h the foci have disappeared from the chromatin.
UniProt Gene Name
At1g08880
UniProt Protein Name
Probable histone H2AXa
UniProt Synonym Protein Names
HTA5
UniProt Primary Accession #
O04848
UniProt Secondary Accession #
Q8LAC0
UniProt Related Accession #
O04848
UniProt Comments
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation .
For research use only, not for clinical use.