Polyubiquitin 12 (UBQ12), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Polyubiquitin 12 (UBQ12)

Product Gene Name

UBQ12 recombinant protein

Product Synonym Gene Name

UBQ12

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MQIFLKTLTG KTKVLEVESS DTIDNVKAKI QDIEGIPPDQ HRLIFAGKQL EDGRTLADYN VQEDSTLHLL LRFRGG

Sequence Positions

1-76, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

25,843 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Polyubiquitin

NCBI Accession #

NP_564675.1

NCBI GI #

18405196

NCBI GenBank Nucleotide #

NM_104380.1

NCBI GeneID

841949

NCBI Official Full Name

ubiquitin 12

NCBI Official Symbol

UBQ12

NCBI Official Synonym Symbols

T7N22.10; ubiquitin 12

NCBI Protein Information

ubiquitin 12

NCBI Summary

Ubiquitin-like gene, believed to be a pseudogene because of amino acid substitutions in 3 of the 5 ubiquitin repeats found in the UBQ12 gene product

UniProt Gene Name

UBQ12

UniProt Protein Name

Polyubiquitin 12

UniProt Primary Accession #

Q3E7K8

UniProt Related Accession #

Q3E7K8

UniProt Comments

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling .