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Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE), Recombinant Protein
Cat: RP00576
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE)
Product Gene Name
FIE recombinant protein
Product Synonym Gene Name
FIE
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MSKITLGNES IVGSLTPSNK KSYKVTNRIQ EGKKPLYAVV FNFLDARFFD VFVTAGGNRI TLYNCLGDGA ISALQSYADE DKEESFYTVS WACGVNGNPY VAAGGVKGII RVIDVNSETI HKSLVGHGDS VNEIRTQPLK PQLVITASKD ESVRLWNVET GICILIFAGA GGHRYEVLSV DFHPSDIYRF ASCGMDTTIK IWSMKEFWTY VEKSFTWTDD PSKFPTKFVQ FPVFTASIHT NYVDCNRWFG DFILSKSVDN EILLWEPQLK ENSPGEGASD VLLRYPVPMC DIWFIKFSCD LHLSSVAIGN QEGKVYVWDL KSCPPVLITK LSHNQSKSVI RQTAMSVDGS TILACCEDGT IWRWDVITK
Sequence Positions
1-369, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
41,257 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Polycomb group protein
NCBI/Uniprot Data
NCBI Accession #
NP_188710.1
NCBI GI #
15232345
NCBI GenBank Nucleotide #
NM_112965.1
NCBI GeneID
821622
NCBI Official Full Name
Transducin/WD40 repeat-like superfamily protein
NCBI Official Symbol
FIE
NCBI Official Synonym Symbols
FERTILIZATION-INDEPENDENT ENDOSPERM; FERTILIZATION-INDEPENDENT ENDOSPERM 1; FIE1; FIS3
NCBI Protein Information
Transducin/WD40 repeat-like superfamily protein
NCBI Summary
Encodes a protein similar to the transcriptional regular of the animal Polycomb group and is involved in regulation of establishment of anterior-posterior polar axis in the endosperm and repression of flowering during vegetative phase. Mutation leads endosperm to develop in the absence of fertilization and flowers to form in seedlings and non-reproductive organs. Also exhibits maternal effect gametophytic lethal phenotype, which is suppressed by hypomethylation. Forms part of a large protein complex that can include VRN2 (VERNALIZATION 2), VIN3 (VERNALIZATION INSENSITIVE 3) and polycomb group proteins FERTILIZATION INDEPENDENT ENDOSPERM (FIE), CURLY LEAF (CLF) and SWINGER (SWN or EZA1). The complex has a role in establishing FLC (FLOWERING LOCUS C) repression during vernalization. In the ovule, the FIE transcript levels increase transiently just after fertilization.
UniProt Gene Name
FIE
UniProt Synonym Gene Names
FIS3
UniProt Protein Name
Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM
UniProt Synonym Protein Names
Protein FERTILIZATION-INDEPENDENT SEED 3
UniProt Primary Accession #
Q9LT47
UniProt Secondary Accession #
Q9XF44
UniProt Related Accession #
Q9LT47
UniProt Comments
Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Required to prevent the proliferation of the central cell by repressing unknown target genes before fertilization. Probably also involved in floral repression mechanism established during early plant development. Regulates the anteroposterior organization of the endosperm. Interacts with the promoter and represses the transcription of genes such as PHE1, that are paternally active and maternally silenced.
For research use only, not for clinical use.