Vector backbone
                                                
                                                
                                                    pMpGWB400                                                
                                             
                                                                                    
                                                
                                                    Modifications to backbone
                                                
                                                
                                                    Coding sequence for a fusion polypeptide between the modified EAR motif plant-specific repression domain showing strong repression activity (SRDX) and glucocorticoid receptor (GR) is cloned downstream of the Gateway cassette.                                                
                                             
                                                                                    
                                                
                                                    Selectable markers
                                                
                                                
                                                    Neomycin (select with G418)                                                
                                             
                                                                                    
                                                
                                                    Bacterial Resistance(s)
                                                
                                                
                                                    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL