Vector backbone
                                                
                                                
                                                    pMpGWB400                                                
                                             
                                                                                    
                                                
                                                    Modifications to backbone
                                                
                                                
                                                    MpEF1α promoter and coding sequence for the modified EAR motif plant-specific repression domain showing strong repression activity (SRDX) are cloned upstream and downstream of the Gateway cassette, respectively.                                                
                                             
                                                                                    
                                                
                                                    Selectable markers
                                                
                                                
                                                    Neomycin (select with G418)                                                
                                             
                                                                                    
                                                
                                                    Bacterial Resistance(s)
                                                
                                                
                                                    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL