Vector backbone
                                                
                                                
                                                    pMpGWB400                                                
                                             
                                                                                    
                                                
                                                    Modifications to backbone
                                                
                                                
                                                    MpEF1α promoter and 3xFLAG coding sequence are cloned upstream and downstream of the Gateway cassette, respectively.                                                
                                             
                                                                                    
                                                
                                                    Selectable markers
                                                
                                                
                                                    Neomycin (select with G418)                                                
                                             
                                                                                    
                                                
                                                    Bacterial Resistance(s)
                                                
                                                
                                                    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL