Total vector size (bp)
                                                
                                                
                                                    11130                                                
                                             
                                                                                    
                                                
                                                    Vector backbone
                                                
                                                
                                                    pMpGWB102                                                
                                             
                                                                                    
                                                
                                                    Modifications to backbone
                                                
                                                
                                                    These PCR-amplified DNA fragments of Citrine-PTS1 were cloned into a pDONR207 plasmid by BP reaction (Invitrogen). The resulting plasmids were mixed with pMpGWB102 vector, and LR reaction (Invitrogen) was performed to generate pMpGWB102-Citrine-PTS1.                                                
                                             
                                                                                    
                                                
                                                    Bacterial Resistance(s)
                                                
                                                
                                                    Spectinomycin, 50 μg/mL